Our PCR genotyping service offers assays for common transgene targets, such as neomycin, luciferase, GFP, lacZ and Cre recombinase, as well as custom gene-specific assay development for targeted mutations (knock-ins/knock-outs). We design two unique assays for each target at no cost to you. DNA is extracted using our proprietary methods and your samples are tested with the two assays to provide definitive results. For targeted mutations, we determine and report on zygosity for each sample. Transgenic samples have presence/absence reported (designated as wild-type or hemizygous – homozygous are not differentiated.) Zygosity of transgenic samples is determined with our higher level zygosity testing service.
Results are GTCAssured with two independent assays for every target. We utilize a proprietary DNA isolation methodology to maximize DNA quality and perform PCR melt curve analysis to generate quality, unambiguous results. Our reports include all methods and raw data for complete evaluation and publication purposes and are delivered in 2-business days guaranteed (allow additional time for new assay development.)
- $7.00 per target, plus $4.00 per additional target
Pricing is per sample, so…
… a single sample with 1 target costs $7
… a single sample with 2 targets costs $7 + $4 = $11
… a single sample with 3 targets costs $7 + $4 + $4 = $15
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*Additional testing not indicated on the original submission, but requested within 24-hours after results are delivered, will be charged at the additional target price. Testing requested beyond 24-hours will be charged at the 1st target price.
Sample Submission Information:
- Strain name
- Mutation design information (for commercially available strains only the vendor information is necessary)
- Strain crossing information (to ensure that our assays do not cross react with other mutations)
- Control samples for targeted mutations. No controls necessary for generic assays