Traditional genotyping requires the use of DNA isolated from harvesting fresh tissue samples from laboratory animals-a procedure known to cause pain and stress to the animal. For most research institutions, it is an ethical and legal obligation to reduce or eliminate pain and distress in research animals. First published in 1959, Russel and Burch’s The Principles of Humane Experimental Technique describes the 3Rs of a new applied science with the intent to improve the treatment of laboratory animals. These terms: replacement, reduction and refinement are the key tenets to guide minimizing animal pain and distress in biomedical research.
Refinement, as defined in the Principles is concerned not only with minimizing distress during experiments, but with maximizing comfort and wellbeing of the animals in husbandry. Eliminating the need for painful tissue excision for the purposes of genotyping is one way to meet this ethical obligation when working with research animals. To this end, we have expanded our genotyping platform to include the ability to screen DNAs isolated from client submitted buccal swabs, a non-invasive procedure that can allow for re-sampling of mice in the absence of painful tissue excision.
For new assays requiring validation, please ship a confirmed positive control (tissue sample) first. DO NOT ship any buccal swab samples until the assay has been validated. We will notify you when the assay has been validated and swab samples can be shipped.
For assays that have already been validated, you may ship swabs to our laboratory and the results will be delivered within 2-3 business days.
Qualitative PCR assessment of transgenics (indicating the presence or absence of a transgene) can be performed with DNA isolated from buccal swabs. However, this platform is not yet optimized for quantitative PCR to determine zygosity (i.e., hemizygous, homozygous, or negative genotype) of transgenes.
All other targeted mutations, point mutations, and endonuclease mediated mutations can be screened using DNA isolated from buccal swabs.
GenoTyping Center of America
10 Water Street, Suite 215
Waterville, ME 04901